A new subspecies, Dendrobaena orientalis karak ssp. nov., is described, and four other earthworm species (Eisenia fetida (Savigny, 1826), Octodrilus transpadanus (Rosa, 1884) Microscolex dubius (Fletcher, 1887) and Ocnerodrilus occidentalis Eisen, 1878 (families Lumbricidae, Acanthodrilidae, Ocnerodrilidae respectively)) are recorded as new for the fauna of Jordan. O. transpadanus also represents a new record for the Levantine zoogeographic province. In addition, new data on the distribution of other earthworm taxa in Jordan are presented. The earthworm fauna of Jordan comprises altogether 13 species, of which 7 are thought to be introduced.